BACKGROUND: The study of ascidians (Chordata. Tunicata) has made a considerable contribution to our understanding of the origin and evolution of basal chordates. To provide further information to support forward genetics in Ciona intestinalis. we used a combination of natural variation and neutral population genetics as an approach for the systematic identification of new mutations. https://www.roneverhart.com/HP-EliteDisplay-S14-14-FHD-LED-Monitor-1080p-Portable-Display-60Hz-5ms-16-9-200-cd-m-700-1-USB-C-3HX46A8/